Crystalline glucose-6-phosphate dehydrogenase was significantly inhibited by L-quinquestriatus venom. Neither boiling nor dialysis could abolish this inhibition completely. Raising the concentration of either G6P or NADP in the reaction mixture reversed the inhibition, indicating that the latter was competitive with respect to the substrate and the coenzyme. An acute, severe febrile reaction with leukocytosis followed vaccination with polyvalent pneumococcal polysaccharide vaccine in a splenectomized patient with myeloproliferative disease.
The 31P-NMR technique was used for the monitoring of intracellular pH and studying its heterogeneity in the femoral biceps muscle of Rana catesbiana under anaerobic conditions. The value of intracellular pH of fresh muscle calculated from the chemical shift of intracellular inorganic phosphate (P1) was 7.3 on average and the line width of P1 was about 0.2 ppm. A linear correlation between the weight of the left-heart ventricle and the systolic blood pressure was found in animals treated with Pindolol as well as in untreated Goldblatt rats. As the line width determined by the relaxation mechanism was 0.099 ppm, the P1 signal in fresh muscle was concluded to consist of overlapped narrow components, which indicated the heterogeneity of muscular pH (about 0.2 pH unit).
Living muscle showed gradual acidification due to glycolysis and the decrease in heterogeneity. When glycolysis was suppressed by iodoacetic acid, slight alkalization due to the breakdown of creatine phosphate was observed. When the Lohmann reaction was suppressed by 2, 4-dinitro-1-fluorobenzene, rapid acidification accompanied by the appearance of a new acidic component was observed with the onset of ATP decrease.
After simple SPV initially a marked decrease of gastric volume and normal emptying into the duodenum were found. With additional pyloric stenosis no significant change was found. The pyloroplasty lead to an increase of gastric volume and delayed emptying. Effects of alanine and glycine on the mechanical and electrical activities of guinea-pig taenia coli were investigated. Alanine (0.1--10 mM) and glycine (0.5--10 mM) produced a dose dependent contraction of guinea-pig taenia coli.
This new component was not detected in the muscle pretreated with glycerol to disrupt the transverse tubules. Therefore, it is likely that this new acidic component originates in the intracellular compartment, and not in the cellular difference. Rats with experimental renovascular hypertension were treated with high doses of beta-blocking agents.
Pindolol 10 mg/kg per day increased Goldblatt-type hypertension, whereas Propranolol 100 mg/kg per day showed an antihypertensive effect. On the contrary, the weights of the left-heart ventricle were significantly higher in those animals treated with Propranolol than in the other groups.
The results show that Pindolol in high doses, possibly on account of its sympathomimetic activity, leads to an increase in Goldblatt hypertension, but does not influence the pressure-dependent progression of left-heart hypertrophy. On the other hand, Propranolol, possibly on account of a cardiodepressory effect, leads to a decrease in blood pressure.
The stimulating effects of alanine and glycine were not inhibited in the presence of atropine (5 muM), tetrodotoxin (0.1 micrograms/ml), diphenhydramine (4 muM), methysergide (3 muM), strychnine (10 muM), and were not influenced by treatment with indomethacin (3 muM). However, these effects were inhibited in the presence of a Ca antagonist, verapamil (10 muM). When the electrical activities of the taenia coli were recorded by the single sucrose-gap method, alanine and glycine (5--10 mM) produced a reduction of membrane potential and increased spike heights and frequencies of action potential.
In LiCl or Choline-Cl and in Na-isethionate solutions, the stimulating effect of alanine was not abolished, but was completely inhibited in KCl-depolarized preparation. After a liquid test meal the motoric and secretory function of the stomach were examined simultaneously by a modified method of intestinal perfusion and aspiration.
From these results, it is considered that both alanine and glycine may directly produce contraction by a depolarization of the cell membrane of guinea-pig taenia coli. In conscious fullgrown minipigs simple SPV alone, SPV and pyloric stenosis and SPV and pyloroplasty were performed. The acid secretion after feeding reduced by SPV was not changed significantly neither by pyloroplasty nor by pyloric stenosis. The baseline values of serum gastrin were elevated after SPV as well as after SPV in combination with pyloric stenosis or pyloroplasty. After food stimulation there was a delayed increase of gastrin after SPV which differed from that after SPV with pyloric stenosis or pyloroplasty only during the first hour. These results show that after SPV no further improvement of the motoric and secretory function can be achieved by an additional pyloroplasty.